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vectrashield plus dapi  (Vector Laboratories)


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    Vector Laboratories vectrashield plus dapi
    Morphological changes of prostate cancer cells exposed to α -tocopheryl succinate ( α -TOS) and vitamin K3 (VK3) plus ascorbic acid (AA) alone or in combination. ( A ) For the soft-agar colony-forming assay, cells were seeded in 24-well culture plates at 10 4 per well in the RPMI-1640 medium containing 0.35% low melting point (LMP) agarose overlaid with 0.7% LMP agarose. The cells were maintained at 37°C in 5% CO 2 for 30 days; every 7 days, 500 μ l of fresh medium was added to each well. The formed colonies were treated with a sub-lethal dose of α -TOS (30 μ M ) or VK3 (3 μ M ) plus AA (0.4 m M ), or the three drugs at these doses together, and the cells were stained with crystal violet after 7 days and inspected by optical microscope. ( B ) PC3 cells were placed overnight in 35-mm dishes on glass coverslips. After a 6-h incubation with α -TOS (30 μ M ) or VK3 (3 μ M ) plus AA (0.4 m M ), or the three drugs at these concentrations together, the cells were stained with phalloidine–TRIC, and the coverslips mounted on microscope slides with <t>Vectrashield</t> containing <t>DAPI</t> and inspected in a fluorescence microscope.
    Vectrashield Plus Dapi, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vectrashield plus dapi/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
    vectrashield plus dapi - by Bioz Stars, 2026-04
    86/100 stars

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    1) Product Images from "α -Tocopheryl succinate promotes selective cell death induced by vitamin K3 in combination with ascorbate"

    Article Title: α -Tocopheryl succinate promotes selective cell death induced by vitamin K3 in combination with ascorbate

    Journal: British Journal of Cancer

    doi: 10.1038/sj.bjc.6605617

    Morphological changes of prostate cancer cells exposed to α -tocopheryl succinate ( α -TOS) and vitamin K3 (VK3) plus ascorbic acid (AA) alone or in combination. ( A ) For the soft-agar colony-forming assay, cells were seeded in 24-well culture plates at 10 4 per well in the RPMI-1640 medium containing 0.35% low melting point (LMP) agarose overlaid with 0.7% LMP agarose. The cells were maintained at 37°C in 5% CO 2 for 30 days; every 7 days, 500 μ l of fresh medium was added to each well. The formed colonies were treated with a sub-lethal dose of α -TOS (30 μ M ) or VK3 (3 μ M ) plus AA (0.4 m M ), or the three drugs at these doses together, and the cells were stained with crystal violet after 7 days and inspected by optical microscope. ( B ) PC3 cells were placed overnight in 35-mm dishes on glass coverslips. After a 6-h incubation with α -TOS (30 μ M ) or VK3 (3 μ M ) plus AA (0.4 m M ), or the three drugs at these concentrations together, the cells were stained with phalloidine–TRIC, and the coverslips mounted on microscope slides with Vectrashield containing DAPI and inspected in a fluorescence microscope.
    Figure Legend Snippet: Morphological changes of prostate cancer cells exposed to α -tocopheryl succinate ( α -TOS) and vitamin K3 (VK3) plus ascorbic acid (AA) alone or in combination. ( A ) For the soft-agar colony-forming assay, cells were seeded in 24-well culture plates at 10 4 per well in the RPMI-1640 medium containing 0.35% low melting point (LMP) agarose overlaid with 0.7% LMP agarose. The cells were maintained at 37°C in 5% CO 2 for 30 days; every 7 days, 500 μ l of fresh medium was added to each well. The formed colonies were treated with a sub-lethal dose of α -TOS (30 μ M ) or VK3 (3 μ M ) plus AA (0.4 m M ), or the three drugs at these doses together, and the cells were stained with crystal violet after 7 days and inspected by optical microscope. ( B ) PC3 cells were placed overnight in 35-mm dishes on glass coverslips. After a 6-h incubation with α -TOS (30 μ M ) or VK3 (3 μ M ) plus AA (0.4 m M ), or the three drugs at these concentrations together, the cells were stained with phalloidine–TRIC, and the coverslips mounted on microscope slides with Vectrashield containing DAPI and inspected in a fluorescence microscope.

    Techniques Used: Staining, Microscopy, Incubation, Fluorescence



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    vectrashield plus dapi  (Vector Laboratories)
    86
    Vector Laboratories vectrashield plus dapi
    Morphological changes of prostate cancer cells exposed to α -tocopheryl succinate ( α -TOS) and vitamin K3 (VK3) plus ascorbic acid (AA) alone or in combination. ( A ) For the soft-agar colony-forming assay, cells were seeded in 24-well culture plates at 10 4 per well in the RPMI-1640 medium containing 0.35% low melting point (LMP) agarose overlaid with 0.7% LMP agarose. The cells were maintained at 37°C in 5% CO 2 for 30 days; every 7 days, 500 μ l of fresh medium was added to each well. The formed colonies were treated with a sub-lethal dose of α -TOS (30 μ M ) or VK3 (3 μ M ) plus AA (0.4 m M ), or the three drugs at these doses together, and the cells were stained with crystal violet after 7 days and inspected by optical microscope. ( B ) PC3 cells were placed overnight in 35-mm dishes on glass coverslips. After a 6-h incubation with α -TOS (30 μ M ) or VK3 (3 μ M ) plus AA (0.4 m M ), or the three drugs at these concentrations together, the cells were stained with phalloidine–TRIC, and the coverslips mounted on microscope slides with <t>Vectrashield</t> containing <t>DAPI</t> and inspected in a fluorescence microscope.
    Vectrashield Plus Dapi, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vectrashield plus dapi/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
    vectrashield plus dapi - by Bioz Stars, 2026-04
    86/100 stars
    		  Buy from Supplier

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    Morphological changes of prostate cancer cells exposed to α -tocopheryl succinate ( α -TOS) and vitamin K3 (VK3) plus ascorbic acid (AA) alone or in combination. ( A ) For the soft-agar colony-forming assay, cells were seeded in 24-well culture plates at 10 4 per well in the RPMI-1640 medium containing 0.35% low melting point (LMP) agarose overlaid with 0.7% LMP agarose. The cells were maintained at 37°C in 5% CO 2 for 30 days; every 7 days, 500 μ l of fresh medium was added to each well. The formed colonies were treated with a sub-lethal dose of α -TOS (30 μ M ) or VK3 (3 μ M ) plus AA (0.4 m M ), or the three drugs at these doses together, and the cells were stained with crystal violet after 7 days and inspected by optical microscope. ( B ) PC3 cells were placed overnight in 35-mm dishes on glass coverslips. After a 6-h incubation with α -TOS (30 μ M ) or VK3 (3 μ M ) plus AA (0.4 m M ), or the three drugs at these concentrations together, the cells were stained with phalloidine–TRIC, and the coverslips mounted on microscope slides with Vectrashield containing DAPI and inspected in a fluorescence microscope.

    Journal: British Journal of Cancer

    Article Title: α -Tocopheryl succinate promotes selective cell death induced by vitamin K3 in combination with ascorbate

    doi: 10.1038/sj.bjc.6605617

    Figure Lengend Snippet: Morphological changes of prostate cancer cells exposed to α -tocopheryl succinate ( α -TOS) and vitamin K3 (VK3) plus ascorbic acid (AA) alone or in combination. ( A ) For the soft-agar colony-forming assay, cells were seeded in 24-well culture plates at 10 4 per well in the RPMI-1640 medium containing 0.35% low melting point (LMP) agarose overlaid with 0.7% LMP agarose. The cells were maintained at 37°C in 5% CO 2 for 30 days; every 7 days, 500 μ l of fresh medium was added to each well. The formed colonies were treated with a sub-lethal dose of α -TOS (30 μ M ) or VK3 (3 μ M ) plus AA (0.4 m M ), or the three drugs at these doses together, and the cells were stained with crystal violet after 7 days and inspected by optical microscope. ( B ) PC3 cells were placed overnight in 35-mm dishes on glass coverslips. After a 6-h incubation with α -TOS (30 μ M ) or VK3 (3 μ M ) plus AA (0.4 m M ), or the three drugs at these concentrations together, the cells were stained with phalloidine–TRIC, and the coverslips mounted on microscope slides with Vectrashield containing DAPI and inspected in a fluorescence microscope.

    Article Snippet: The cells were then incubated with TRIC-conjugated phalloidine (Sigma) (2 μ g ml −1 ) at room temperature for 30 min, and the coverslips mounted on microscope slides with VectraShield plus DAPI (Vector Laboratories, Burlingame, CA, USA) and inspected in a fluorescence microscope (Zeiss, Axiocam MRc5, Thomwood, NY, USA, magnification × 60).

    Techniques: Staining, Microscopy, Incubation, Fluorescence